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LED470-DF36W
470 nm Blue LED Transilluminator

High-intensity dark-field LED transilluminator optimized for blue-light excitation around 470 nm

The LED470-DF36W is a 470 nm blue LED transilluminator designed for safe,
UV-free fluorescence gel imaging.

It provides efficient excitation for blue-light excitable nucleic acid stains, enabling high-contrast DNA/RNA gel observation with dyes such as SYBR Green, SYBR Safe, GelGreen and Midori Green.

This model is suitable for standard UV-free nucleic acid gel imaging workflows where 470 nm blue-light excitation is preferred.

470 nm Blue LED 36W Class Dark-Field Dichroic Filter UV-Free Gel Cutting

LED470-DF36W

LED470-DF36W 470 nm blue LED transilluminator for gel imaging
  • 470 nm visible blue LED excitation for UV-free gel imaging.
  • Dark-field illumination helps reduce direct stray light into the camera or observer.
  • Dichroic filter system suppresses unwanted long-wavelength background light.
  • Suitable for DNA, RNA and electrophoresis gel observation, imaging and gel cutting.
  • Recommended for SYBR Green, SYBR Safe, GelGreen, Midori Green and similar blue-light excitable nucleic acid stains.

Set Composition & Price

LED470-DF36W set image
  • LED Transilluminator with high-contrast sharp-cut filter cover, cuts off 560 nm or below.
  • AC-DC Adapter 100-240 V universal input, 24 V 2.7 A 65 W output.
Product Item Code Price
Dark-field LED Transilluminator, 470 nm blue LED LED470-DF36W US$ 950

High-Intensity LED Arrangement

The LED470-DF36W uses 24 high-intensity chip-type LEDs arranged on both sides of the luminous surface.

This structure provides strong blue-light excitation while helping keep direct light away from the observer and camera.


Dark-Field Illumination System

The dark-field illumination system directs light from the lower left and right sides of the illuminator.

Because the excitation light does not directly enter the camera or observer, fluorescence can be observed with improved contrast.


Dichroic Filter Effect

The dichroic filter cuts unwanted long-wavelength components from the LED light.

Together with the light-absorbing mat, it helps suppress background glow and improve the visibility of weak fluorescence bands.


Removable Orange Observation Cover

The high-contrast orange sharp-cut cover can be attached and removed easily.

It allows observation and gel cutting while reducing excitation light below 560 nm.

Compatible with Pict LaBox and Bio-Pyramid Mini

Pict LaBox Compatible

The LED470-DF36W can be combined with Pict LaBox for gel observation, cutting and image documentation using a digital camera or CCD camera system.

Bio-Pyramid Mini Compatible

The compact Bio-Pyramid Mini can be installed stably on the LED470-DF36W for mini-gel fluorescence imaging and documentation.

LED470 or LED505? Which Model Should You Choose?

Selection Guide Choose LED470-DF36W for blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. Choose LED505-DF36W for longer-wavelength green-light excitation dyes such as EtBr, GelRed and SYBR Gold. For uncertain staining conditions, please check the excitation/emission spectrum of your dye.

View LED505-DF36W View Filter Guide

Related Gel Documentation Resources

Bio-Pyramid System

LED470-DF36W with Bio-Pyramid Mini gel documentation system

Complete gel documentation hood for imaging and analysis.

Details

Filter Selection Guide

Gel documentation filter guide

Choose the correct emission filter for your gel stain.

Guide

Sample Images

Gel documentation sample images

See real gel imaging results and fluorescence examples.

Samples

Specification

Model LED470-DF36W
Peak Wavelength Blue 470 nm
Luminous Area 140 x 130 mm effective area
LED 24 high-intensity chip-type LEDs arranged in parallel
Illumination Method Dark-field illumination from diagonally below the left and right sides
Background Suppression Multi-layer dichroic filters installed directly on the LED
to suppress long-wavelength background light
Observation Cover Filter Orange cast acrylic cover, cuts off 560 nm or below
Compatible Fluorescent Reagents DNA / RNA stains optimized for blue-light excitation:
SYBR Green, SYBR Safe, GelGreen, Midori Green

Recommended excitation range:
Blue-light excitable nucleic acid stains with excitation or
absorption peaks around 450-490 nm
Power Supply Input: 100-240 V, 50/60 Hz universal /
Output: 24 V, 2.7 A, 65 W / PSE, UL and CE certified
Power Consumption 32 W constant
LED Lifetime Approx. 40,000 hours
Cooling Forced-air cooling by fan
Accessories AC-DC adapter, instruction manual
Body Size / Weight W290 x D230 x H100 mm, H120 mm including cover
Approx. 2.2 kg
What is LED470-DF36WLED470-DF36W is a 470 nm blue LED transilluminator designed for safe, UV-free DNA/RNA gel imaging. It uses dark-field illumination and a dichroic filter system to enhance fluorescence contrast with blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green.

FAQ - LED470-DF36W

What is LED470-DF36W used for? It is used for UV-free fluorescence excitation in DNA, RNA and electrophoresis gel imaging workflows using 470 nm blue-light excitation.
Which stains are suitable for this model? It is suitable for blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen, Midori Green and similar dyes with excitation or absorption peaks around 450-490 nm.
What is the difference between 470 nm and 505 nm models? 470 nm models are optimized for blue-light excitable stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. 505 nm models provide better excitation efficiency for longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.
Do I need an emission filter? Yes. A suitable emission filter is recommended for clear fluorescence imaging. The best filter depends on the stain, camera and imaging workflow.
Need help selecting the right LED transilluminator? Please tell us your gel stain, gel size, camera system, emission filter and imaging workflow.
We will recommend a suitable wavelength and model configuration.