The LED470-DF36W is a 470 nm blue LED transilluminator designed for safe,
UV-free fluorescence gel imaging.
It provides efficient excitation for blue-light excitable nucleic acid stains, enabling high-contrast DNA/RNA gel observation with dyes such as SYBR Green, SYBR Safe, GelGreen and Midori Green.
This model is suitable for standard UV-free nucleic acid gel imaging workflows where 470 nm blue-light excitation is preferred.
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| Product | Item Code | Price |
|---|---|---|
| Dark-field LED Transilluminator, 470 nm blue LED | LED470-DF36W | US$ 950 |
The LED470-DF36W uses 24 high-intensity chip-type LEDs arranged on both sides of the luminous surface.
This structure provides strong blue-light excitation while helping keep direct light away from the observer and camera.
The dark-field illumination system directs light from the lower left and right sides of the illuminator.
Because the excitation light does not directly enter the camera or observer, fluorescence can be observed with improved contrast.
The dichroic filter cuts unwanted long-wavelength components from the LED light.
Together with the light-absorbing mat, it helps suppress background glow and improve the visibility of weak fluorescence bands.
The high-contrast orange sharp-cut cover can be attached and removed easily.
It allows observation and gel cutting while reducing excitation light below 560 nm.
The LED470-DF36W can be combined with Pict LaBox for gel observation, cutting and image documentation using a digital camera or CCD camera system.
| Model | LED470-DF36W |
|---|---|
| Peak Wavelength | Blue 470 nm |
| Luminous Area | 140 x 130 mm effective area |
| LED | 24 high-intensity chip-type LEDs arranged in parallel |
| Illumination Method | Dark-field illumination from diagonally below the left and right sides |
| Background Suppression | Multi-layer dichroic filters installed directly on the LED to suppress long-wavelength background light |
| Observation Cover Filter | Orange cast acrylic cover, cuts off 560 nm or below |
| Compatible Fluorescent Reagents | DNA / RNA stains optimized for blue-light excitation: SYBR Green, SYBR Safe, GelGreen, Midori Green Recommended excitation range: Blue-light excitable nucleic acid stains with excitation or absorption peaks around 450-490 nm |
| Power Supply | Input: 100-240 V, 50/60 Hz universal / Output: 24 V, 2.7 A, 65 W / PSE, UL and CE certified |
| Power Consumption | 32 W constant |
| LED Lifetime | Approx. 40,000 hours |
| Cooling | Forced-air cooling by fan |
| Accessories | AC-DC adapter, instruction manual |
| Body Size / Weight | W290 x D230 x H100 mm, H120 mm including cover Approx. 2.2 kg |
| What is LED470-DF36W used for? | It is used for UV-free fluorescence excitation in DNA, RNA and electrophoresis gel imaging workflows using 470 nm blue-light excitation. |
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| Which stains are suitable for this model? | It is suitable for blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen, Midori Green and similar dyes with excitation or absorption peaks around 450-490 nm. |
| What is the difference between 470 nm and 505 nm models? | 470 nm models are optimized for blue-light excitable stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. 505 nm models provide better excitation efficiency for longer-wavelength dyes such as EtBr, GelRed and SYBR Gold. |
| Do I need an emission filter? | Yes. A suitable emission filter is recommended for clear fluorescence imaging. The best filter depends on the stain, camera and imaging workflow. |