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LED Light for Fluorescence Excitation

UV, visible and IR LED light sources for fluorescence observation,
microscopy and gel documentation

Long-life, low-heat and energy-efficient LED light sources are useful for fluorescence excitation in laboratory observation, microscope imaging and gel documentation workflows.

Mecan USA offers LED transmitted light sources, flexible LED epi-lights, handheld LED flashlights, microscope filter holder options and reagent guidance. Suitable wavelength and filter selection should be matched to the fluorescent dye, sample and imaging setup.

LED light sources for fluorescence excitation
Fluorescence Excitation Microscope Gel Documentation Filter / Reagent Matching

LED Fluorescence Excitation Product Lineup

LED Transmitted Light LED transmitted light for fluorescence excitation
Transmitted · Fluorescence
LED transmitted light sources for fluorescent samples, microscope observation and gel documentation workflows. They can be selected according to the sample, dye and wavelength requirement.
LED Epi-Light Flexible LED epi-light for fluorescence observation
Epi · Double Arm
Flexible double-arm LED epi-light for macro and microscope observation. Useful when top illumination angle and working distance must be adjusted around the specimen.
LED Flashlight Handheld LED flashlight for fluorescence checking
Handheld · UV to IR
Handheld LED flashlights with wavelength options from UV to IR. Useful for quick fluorescence checking, field observation and simple optical inspection.
Microscope Filter Holder Microscope filter holder for fluorescence observation
Filter · Fluorescence
Filter holder options for building a simple fluorescence observation setup with a stereo microscope or imaging system. Proper excitation and emission filter matching is required.

LED Light Sources for Fluorescence Observation

  • LED transmitted light for fluorescent samples, gels and microscope observation.
  • Flexible LED epi-light for top illumination and multi-angle fluorescence checking.
  • Handheld LED flashlights available in UV to IR wavelength ranges.
  • Microscope filter holder options for simple fluorescence observation setups.
  • Useful for fluorescence reagents such as SYBR Green, SYBR Gold, GelRed, GelGreen, FITC, GFP and related dyes.
  • Low heat generation helps reduce sample heating compared with some conventional light sources.
  • Compact light sources can be combined with suitable emission filters and imaging devices.
  • Selection depends on wavelength, sample type, filter combination and observation geometry.
Important: Fluorescence performance depends on the excitation wavelength, emission filter, sample concentration, camera sensitivity and surrounding light conditions. Please confirm the reagent and optical setup before selecting a light source.

How to Choose

Use the steps below as a starting point for selecting a suitable LED fluorescence excitation setup.

  1. STEP 1 — Check the reagent and excitation wavelength. Look up your reagent or fluorophore in the Reagent Guide to find a suitable excitation wavelength.
  2. STEP 2 — Review the illumination options. Mecan USA offers transmitted LED fluorescence excitation (TR8W, DF36W, TR60W) and epi LED fluorescence excitation (HDA, SDA) for use with existing compatible stereo microscopes; review each product line based on your setup and observation requirements.
  3. STEP 3 — Confirm filter requirements. Fluorescence observation requires a suitable emission filter matched to the light source and reagent. Some models include a filter, while others are used with a separate microscope filter holder, as described on each product page.

Filter and Reagent Support

When selecting an LED light source for fluorescence excitation, the reagent, wavelength and filter combination should be checked together. The filter guide below can help narrow down suitable combinations for gel documentation and fluorescence imaging applications.

LED Color Selection Guide
Need help selecting a wavelength or filter combination? Please contact us with your reagent name, sample type, microscope or imaging system model and intended observation method.

Frequently Asked Questions

Q What are LED light sources used for in fluorescence excitation? ▼
A LED light sources are used to excite fluorescent dyes, reagents and samples so that emitted fluorescence can be observed or photographed with an appropriate filter combination.
Q Can LED fluorescence lights be used with microscopes? ▼
A Yes. Depending on the wavelength, light geometry and filter configuration, LED transmitted light, LED epi-light and microscope filter holders can be used for fluorescence observation with suitable microscopes.
Q Which wavelength should be selected? ▼
A The proper wavelength depends on the excitation range of the fluorescent reagent or sample, such as SYBR Green, SYBR Gold, GelRed, GelGreen, FITC, GFP, DsRed or other dyes. Filter selection is also important for clean fluorescence observation.
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