Filter Guide for Various Fluorescent Reagent (Ex/Em)
| List of Reagents ・ Ex/Em wavelengths ・ LED light source ・ Filterwork |
Following are list of most popular reagents we often receive inquiry.
Click Spectral button for filterwork guide of each reagent. |
| Reagent |
EX nm |
EX
(Sub) |
EM nm |
spectral
info |
|
Reagent |
EX nm |
EX
(Sub) |
EM nm |
spectral
info |
| Alexa Fluor 532 |
532 |
|
554 |
 |
mCitrine |
516 |
|
529 |
|
| Cy3 |
550 |
|
570 |
|
Midori Green |
490 |
290 |
530 |
|
| DsRed |
557 |
|
579 |
|
SYBR Gold |
495 |
|
540 |
|
| EtBr |
300 |
518 |
605 |
|
SYBR Green I |
498 |
|
522 |
|
| FITC |
490 |
|
525 |
|
SYPRO Orange |
470 |
|
570 |
|
| Flamingo |
512 |
271 |
535 |
|
SYPRO Red |
550 |
300 |
630 |
|
| Gel Green |
250 |
500 |
530 |
|
SYPRO Ruby |
280 |
450 |
620 |
|
| Gel Red |
270 |
510 |
600 |
|
TagRFP |
555 |
|
583 |
|
| GFP |
488 |
|
507 |
|
tdTomato |
554 |
|
581 |
|
| mCherry |
580 |
|
610 |
|
YFP |
513 |
|
527 |
|
|
| Ex/Em list for other reagents ⇒ |
 |
| Notice : |
The guide is reference purpose only. It does not guarantee visibility of
any certain emission. Result may vary depends on such various factors as
LED brightness, filter quality, and camera specifications, etc. |
|
Symbol Meaning |
 |
Most suitable |
| * |
Most suitable but a long-cut filter may be required to cut the background |
 |
Suitable |
 |
Usable but with limited effect compared to and |
 |
Not fit for use |
★ Place pointer above and see LED/Filter wavelengths |
For cellular labeling in fluorescence microscopy and in cell biology.
Retains sulfonic acid which is
directly connected to the fluorescent. Especially bright with rhodamine
derivative. The reagent with great
light stability |
| Life Technology |
 |
|
Em/Ex are too close and difficult to separate absorption & excitation spectra. 505nm/SC-56 pair is effective. Filter to cut long
wavelength side is required to eliminate background when used
with transmitted light source. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
 |
|
|
| 505nm |
 |
* |
|
| 530nm |
 |
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
In addition to such biological assays as immunocytochemistry, Cyanine
dye fluorescence reagent is often
used for labelling RNA. |
| Mirus Bio LLC |
|
|
Em/Ex are close and difficult to separate absorption & excitation spectra.
505nm & SC-56 pair is effective. Filter to cut long
wavelength side is required to eliminate background when used
with transmitted light source. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
Exhibits strong and stable
expression in bacterial cells
with minimal toxicity. |
| Clontech Laboratories, Inc |
|
|
Em/Ex peaks are close but both lines are spread over wide wavelength and easier to separate. Blue, Cyan, Green LED can be used for excitation and
red filter is effective for separation. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
The most widely used reagent for band staining. Treated as hazardous waste
by many organizations and require special care for handling. |
| Wikipedia |
|
|
It has Ex peak at both 300/518nm.
LED505/530nm can be used but efficiency is not high. High power light source
and effective filter is required to eliminate background. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
Dramatic Effect of
Dichroic Filter Effect ⇒ |
 |
|
* Mecan LED transilluminator is equipped with high quality dichroic
filter LC525 |
|
LED505-TR60W
 |
|
★ Place pointer above and see LED/Filter wavelengths |
A type of fluorochrome widely used for microscopic observation.
Often utilized for bloodstain search, dye tracking in the area of forensic
medicine and serology |
| Kirkegaard & Perry Lab |
|
|
| Ex peak at 490nm and can be excited with 505nm LED. However Ex/Em is close
and may require LED device with background eliminating features. High power
blue LED with SC52 filter can also be effective. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
|
* 470nm + SC52 pair is also effective |
★ Place pointer above and see LED/Filter wavelengths |
Low cost protein detection with simple protocol. High sensitivity and quantitatively
enables identification
of protein by mass spectrometry. Compatible also with UV excitation. |
| Bio-Rad |
|
|
Ex 512nm and LED505nm can be effective. LED device with background eliminating
feature or high power
blue LED & SC52 pair can also be effective since Ex/Em is close. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
 |
|
|
|
|
* 470nm + SC52 pair is also effective |
★ Place pointer above and see LED/Filter wavelengths |
Green fluorescent nucleic acid dye designed to replace the highly toxic
EtBr. Compare to EtBr, it is ultra-sensitive and very stable, both hydrologically
and thermally. Very simple procedures for pre or post staining |
| cBiotium |
|
|
| Wavelength characteristic is similar to FITC, and so is filterworks. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
|
* 470nm + SC52 pair is also effective |
★ Place pointer above and see LED/Filter wavelengths |
Fluorescent nucleic acid dye designed to replace the highly toxic EtBr. Compare to EtBr, it is ultra-sensitive and very stable, both hydrologically and thermally. Very simple procedures for pre or post staining. |
| cBiotium |
|
|
Wavelength characteristic is similar to EtBr. Compare to EtBr, it has more abosrption around 520nm and thus
LED light is effective. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
Enables real time detection without damaging cell. It also demonstrate
its function as fusion protein (GFP tag) Widely used as a tool to locate
protein which is taking part in
signal transaction inside the cell. |
| GE healthcare |
|
|
Em/Ex peaks are close but Em is spread over to short wavelength
side and Blue LED is effective |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
Red fluorescent proteins isolated
from Discosoma sp. Utilizes for fusion expression with target protein,
promoter activity monitoring and bacteria expression. Takes short time
for expression and stable as well. |
| Clontech Laboratories, Inc |
|
|
| Em/Ex peaks are close but Em is spread over to short wavelength side and
Cyan or Green LED can be used. Excitation with Green LED and separation
with red filter is effective. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
mCitrine is an empty vector and gene insertion can be achieved by LIC cloning
protocol. mCitrine is highly versatile with its compatibility with many
other vector as well as usability as single expression vector. |
| ? ? ? |
|
|
Ex 510m and 505nm LED is efficient. However Ex/Em is close and may require
LED device with background eliminating features. High power blue LED with
SC52 filter is also effective.
|
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
|
* 470nm + SC52 pair is also effective |
★ Place pointer above and see LED/Filter wavelengths |
Ideal Nucleic acid staining reagent for prestained method. Low background
and high S/N ratio makes safe alternative to the ethidium bromide. |
| Fast Gene |
|
|
| Ralatively easy to excite with Blue LED (470m). Easy to separate since
Ex/Em peak has some space between them. |
| Ex |
Effcy |
Filter evaluation |
| SC-54 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
|
|
| 530nm |
|
|
|
|
|
* SC52 is also effective |
★ Place pointer above and see LED/Filter wavelengths |
|
| Life Technology |
|
|
Relatively easy to excite with Blue
and Cyan LED. Easy to separate since Ex/Em peak has some space between
them. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
Asymmetrical cyanine dye used as
a nucleic acid stain in molecular biology. It binds to double helix DNA
and can be replacement for EtBr as it is safer to work However any small
molecule capable of binding DNA with high affinity is a possible carcinogen,
including SYBR Green. |
| Life Technology |
|
|
Ex is similar to SYBR Gold, however Em is adjacent to Ex and bit harder
to separate. LED 505 with background eliminating features or high power
blue LED & SC52 pair is also effective. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
|
* 470nm + SC52 pair is also effective |
★ Place pointer above and see LED/Filter wavelengths |
Provides fast, simple, sensitive staining of proteins. Has very little protein-to-protein variation in staining. Enables long lasting
and stable emission. With low background, it requires less time
for cleaning gel. Detection with immunoreagent is also possible
with western blotting. |
| GE healthcare |
|
|
Ex is spread over to wide range
and both Blue and Cyan LED are
highly effective for excitation.
With enough space between Ex&Em,
it is relatively easy to separate too |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
For cellular labeling in fluorescence microscopy and in cell biology.
Retains sulfonic acid which is
directly connected to the fluorescent. Especially bright with rhodamine derivative. The reagent with great
light stability
|
| GE healthcare |
|
|
Ex is spread over to wide range and both blue and cyan are effective for
excitation. Most emission can transmit both SC-56 & SC-60 and it
is relatively easy to separate as well. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
For cellular labeling in fluorescence microscopy and in cell biology.
Retains sulfonic acid which is
directly connected to the fluorescent. Especially bright with rhodamine derivative. The reagent with great
light stability
|
| GE healthcare |
|
|
With wide space between the peaks
of Ex & Em, it is considered easy reagent for separation. Blue LED
is most effective for excitation.
Cyan LED is also effective. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
Generated from the wild-type RFP from sea anemone entacmaea quadricolor.
It is brightest monomeric red fluorescent protein available. Mammalian
cells transiently transfected with TagRFP expression vectors produce bright
fluorescence
in 10-12 hrs after transfection. |
| Evrogen JSC |
|
|
Cyan, or Green LED is effective for excitation. Em is too close with Green,
and background may appear with SC-56 but sould be able to
separate with SC-60 since Em is
spread over to long wavelength side. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
Exceptionally bright red fluorescent protein (2.5x brighter than EGFP)
It is easily detected as deep as 1 cm below the surface, and ideal for
live animal imaging studies. High cohesion stability make it suitable to
produce protein N-, C- fusion protein too. |
Takara Bio
|
|
|
Wavelength characteristics is similar to Tag RFP and Em/Ex are close.
Cyan LED & shortpass filters pair or Green LED & SC-60 filter pair
should be used to eliminate background. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
★ Place pointer above and see LED/Filter wavelengths |
YFP is synthetic non-aequorea fluorescent proteins that can be amplified/cut by various PCR and cloned into any other expression vector of choice. These vectors can also be used as expression vectors or as positive controls and allow monitoring of inducible protein expression. |
| Cosmo Bio |
|
|
| Ex peak at 512nm and can be excited with 505nm LED. However Ex/Em is close
and may require LED device with background eliminating features. High power
blue LED with SC52 filter can also be effective. |
| Ex |
Effcy |
Filter evaluation |
| SC-56 |
SC-60 |
| 470nm |
|
|
|
| 505nm |
|
* |
|
| 530nm |
|
|
|
|
|
* 470nm + SC52 pair is also effective |
| * |
⇒ Long cut filter may required to eliminate background |
| Ex/Em list for other reagents ⇒ |
|
| Notice : |
The guide is reference purpose only. It does not guarantee visibility of
any certain emission. Result may vary depends on such various factors as
LED brightness, filter quality, and camera specifications, etc. |
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