Gel Imaging / Ex-Em Reference
Fluorescent Reagent Database
Excitation and Emission Wavelengths
A practical reference list for fluorescence reagents used in gel documentation, fluorescence imaging, and emission filter selection. Use the excitation (EX) and emission (EM) wavelengths as a starting point for matching 470 nm LED, 505 nm LED, UV illumination, and filter combinations.
Values are provided as general reference ranges. Actual excitation and emission peaks may vary depending on reagent formulation, binding state, solvent conditions, measurement methods, optical filters, camera sensitivity, exposure settings, and imaging system configuration.
1. Check the Excitation Wavelength
Start with the reagent's EX peak and compare it with the available light source, such as 470 nm LED, 505 nm LED, or UV transillumination.
2. Match the Emission Range
Select an emission filter that passes the reagent's fluorescence signal while blocking excitation light from the LED or UV source.
3. Confirm with Real Samples
Final image quality depends on reagent chemistry, gel thickness, camera settings, and background fluorescence. Test under actual conditions when possible.
Quick Selection Guide by Light Source
- 470 nm LED: useful for many blue-light excitable DNA/RNA dyes and green fluorescent signals.
- 505 nm LED: useful for cyan-green excitation ranges and selected orange/red-emitting dyes.
- UV 312 / 365 nm: useful for traditional UV-excited stains such as EtBr and UV-responsive reagents.
Common Reagents for Gel Imaging
SYBR Green
Common nucleic-acid stain. Use an emission filter that passes green fluorescence while suppressing excitation light.
SYBR Safe
Often used with blue-light or cyan-green excitation. Practical compatibility should be confirmed with the actual gel and filter set.
GelGreen
Green-emitting nucleic-acid dye. A green-pass emission filter is commonly used to improve contrast.
GelRed
Red/orange-emitting nucleic-acid stain. Filter selection should block excitation light while passing red-orange emission.
EtBr
Traditional UV-excited nucleic-acid stain. Use appropriate UV safety precautions and an orange/red emission filter.
GFP / FITC Range
Useful reference range for green fluorescent proteins and fluorescein-type signals. Camera and filter sensitivity are important.
Type a reagent name or wavelength to narrow the list below. The complete alphabetical list is preserved for reference.
Fluorescent Reagent List (Alphabetical Order)
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