LED470-DF36W 470 nm Blue LED Transilluminator
High-intensity dark-field LED transilluminator optimized for blue-light excitation around 470 nm
The LED470-DF36W is a 470 nm blue LED transilluminator designed for safe, UV-free fluorescence gel imaging.
It provides efficient excitation for blue-light excitable nucleic acid stains, enabling high-contrast DNA/RNA gel observation with dyes such as SYBR Green, SYBR Safe, GelGreen and Midori Green.
This model is suitable for standard UV-free nucleic acid gel imaging workflows where 470 nm blue-light excitation is preferred.
- 470 nm Blue LED
- 36W Class
- Dark-Field
- Dichroic Filter
- UV-Free
- Gel Cutting
LED470-DF36W
- 470 nm visible blue LED excitation for UV-free gel imaging.
- Dark-field illumination helps reduce direct stray light into the camera or observer.
- Dichroic filter system suppresses unwanted long-wavelength background light.
- Suitable for DNA, RNA and electrophoresis gel observation, imaging and gel cutting.
- Recommended for SYBR Green, SYBR Safe, GelGreen, Midori Green and similar blue-light excitable nucleic acid stains.
Set Composition & Price
- LED Transilluminator with high-contrast sharp-cut filter cover, cuts off 560 nm or below.
- AC-DC Adapter 100-240 V universal input, 24 V 2.7 A 65 W output.
| Product | Item Code | Price |
|---|---|---|
| Dark-field LED Transilluminator, 470 nm blue LED | LED470-DF36W | US$ 950 |
High-Intensity LED Arrangement
The LED470-DF36W uses 24 high-intensity chip-type LEDs arranged on both sides of the luminous surface.
This structure provides strong blue-light excitation while helping keep direct light away from the observer and camera.
Dark-Field Illumination System
The dark-field illumination system directs light from the lower left and right sides of the illuminator.
Because the excitation light does not directly enter the camera or observer, fluorescence can be observed with improved contrast.
Dichroic Filter Effect
The dichroic filter cuts unwanted long-wavelength components from the LED light.
Together with the light-absorbing mat, it helps suppress background glow and improve the visibility of weak fluorescence bands.
Removable Orange Observation Cover
The high-contrast orange sharp-cut cover can be attached and removed easily.
It allows observation and gel cutting while reducing excitation light below 560 nm.
Compatible with Pict LaBox and Bio-Pyramid Mini
Pict LaBox Compatible
The LED470-DF36W can be combined with Pict LaBox for gel observation, cutting and image documentation using a digital camera or CCD camera system.
Bio-Pyramid Mini Compatible
The compact Bio-Pyramid Mini can be installed stably on the LED470-DF36W for mini-gel fluorescence imaging and documentation.
LED470 or LED505? Which Model Should You Choose?
Selection Guide Choose LED470-DF36W for blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. Choose LED505-DF36W for longer-wavelength green-light excitation dyes such as EtBr, GelRed and SYBR Gold. For uncertain staining conditions, please check the excitation/emission spectrum of your dye.
Related Gel Documentation Resources
Specification
| Model | LED470-DF36W |
|---|---|
| Peak Wavelength | Blue 470 nm |
| Luminous Area | 140 x 130 mm effective area |
| LED | 24 high-intensity chip-type LEDs arranged in parallel |
| Illumination Method | Dark-field illumination from diagonally below the left and right sides |
| Background Suppression | Multi-layer dichroic filters installed directly on the LED to suppress long-wavelength background light |
| Observation Cover Filter | Orange cast acrylic cover, cuts off 560 nm or below |
| Compatible Fluorescent Reagents | DNA / RNA stains optimized for blue-light excitation: SYBR Green, SYBR Safe, GelGreen, Midori Green Recommended excitation range: Blue-light excitable nucleic acid stains with excitation or absorption peaks around 450-490 nm |
| Power Supply | Input: 100-240 V, 50/60 Hz universal / Output: 24 V, 2.7 A, 65 W / PSE, UL and CE certified |
| Power Consumption | 32 W constant |
| LED Lifetime | Approx. 40,000 hours |
| Cooling | Forced-air cooling by fan |
| Accessories | AC-DC adapter, instruction manual |
| Body Size / Weight | W290 x D230 x H100 mm, H120 mm including cover Approx. 2.2 kg |
What is LED470-DF36WLED470-DF36W is a 470 nm blue LED transilluminator designed for safe, UV-free DNA/RNA gel imaging. It uses dark-field illumination and a dichroic filter system to enhance fluorescence contrast with blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green.
FAQ - LED470-DF36W
What is LED470-DF36W used for?
It is used for UV-free fluorescence excitation in DNA, RNA and electrophoresis gel imaging workflows using 470 nm blue-light excitation.
Which stains are suitable for this model?
It is suitable for blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen, Midori Green and similar dyes with excitation or absorption peaks around 450-490 nm.
What is the difference between 470 nm and 505 nm models?
470 nm models are optimized for blue-light excitable stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. 505 nm models provide better excitation efficiency for longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.
Do I need an emission filter?
Yes. A suitable emission filter is recommended for clear fluorescence imaging. The best filter depends on the stain, camera and imaging workflow.
Need help selecting the right LED transilluminator? Please tell us your gel stain, gel size, camera system, emission filter and imaging workflow.
We will recommend a suitable wavelength and model configuration.