What is an LED epi-light used for in fluorescence excitation?

An LED epi-light illuminates the specimen from above or from an oblique angle using a selected excitation wavelength. It is useful for stereo microscope fluorescence observation when flexible arm positioning and wavelength selection are important.

Which wavelength should I choose for fluorescence observation?

The proper wavelength depends on the fluorescent reagent or fluorophore, the specimen, the emission filter and the camera or observation system. 470 nm is commonly used for blue-light excitable dyes, while 505 nm or 530 nm may be selected for longer-wavelength excitation workflows.

What is the difference between HDA and SDA Series LED epi-lights?

The HDA Series is positioned as a higher-intensity twin-arm LED epi-light, while the SDA Series is a compact and cost-effective twin-arm LED light for fluorescence excitation.

Is an emission filter required?

For clear fluorescence observation or imaging, an emission filter matched to the fluorescent dye and observation system is normally required.

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Excitation LED

LED Epi-Light for Fluorescence Excitation

Flexible twin-arm gooseneck LED light sources for stereo microscope fluorescence observation

Mecan LED epi-light sources provide selected excitation wavelengths from above or from an oblique angle. The flexible twin-arm design makes it easy to adjust the illumination angle, working distance and light balance around a stereo microscope or inspection setup.

The lineup includes the high-intensity HDA Series and the compact SDA Series. Select the LED wavelength according to the fluorescent reagent, specimen, emission filter and camera system. For clear fluorescence observation, an appropriate emission filter or filter holder system is usually required.

470 nm Blue LED 505 nm Cyan LED 530 nm Green LED Epi Light Stereo Microscope

HDA Series SDA Series Wavelength Guide Filter Support Filter Guide

LED Epi-Light Lineup

These LED epi-light systems are designed for fluorescence excitation where transmitted illumination is not suitable or where flexible top / side illumination is preferred. They are especially useful for stereo microscope observation, small specimen screening and fluorescence documentation with a suitable emission filter.

HDA Series

High-intensity twin-arm LED epi-light

High-intensity, flexible twin-arm LED lighting for microscope fluorescence excitation. The gooseneck arms allow the illumination angle to be adjusted for specimen shape, working distance and observation contrast.

View HDA Series

SDA Series

Compact twin-arm LED epi-light

Compact and cost-effective LED twin flexible arm light for fluorescence excitation. Suitable for simple stereo microscope fluorescence observation and small laboratory setups where space and budget are important.

View SDA Series

Main models: HDA-TB3 (470 nm), HDA-TG3 (530 nm), SDA-B4 (470 nm), SDA-C4 (505 nm)

Model and Wavelength Selection Guide

Series / Model	LED Wavelength	Positioning	Typical Use
HDA-TB3	470 nm Blue LED	High-intensity HDA Series	Blue-light fluorescence excitation for dyes and markers that respond around the blue region.
HDA-TG3	530 nm Green LED	High-intensity HDA Series	Green-region fluorescence excitation for suitable orange / red emission workflows.
SDA-B4	470 nm Blue LED	Compact SDA Series	Compact blue-light excitation for simple fluorescence observation.
SDA-C4	505 nm Cyan LED	Compact SDA Series	Cyan / green-region excitation for compatible fluorescent stains and observation setups.

Selection point: The best LED wavelength depends on the fluorescent reagent, specimen, excitation spectrum, emission filter and camera sensitivity. Please confirm the dye/filter combination before final selection.

Emission Filter and Observation Notes

Flexible twin-arm positioning helps optimize reflection, shadow and fluorescence contrast.
Oblique epi-light is useful when the specimen cannot be illuminated from below.
For fluorescence imaging, an emission filter matched to the dye is normally required.
Final image quality also depends on microscope optics, camera sensitivity and ambient light control.

Reagent and filter selection reference
for fluorescence observation
and gel documentation workflows.

Filter Guide

Need help selecting an LED epi-light?
Please tell us your microscope model, fluorescent reagent, target wavelength,
emission filter and observation or imaging purpose.
We can help recommend a suitable HDA or SDA configuration.