What is LED505-DF36W used for?

LED505-DF36W is used for UV-free fluorescence excitation in DNA, RNA and electrophoresis gel imaging workflows using 505 nm green-light excitation, especially with longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.

Which stains are suitable for LED505-DF36W?

LED505-DF36W is optimized for nucleic acid stains with excitation or absorption peaks around 490-510 nm, such as EtBr, GelRed and SYBR Gold. It is also compatible with selected protein stains such as SYPRO Red and Flamingo, and fluorescent proteins such as DsRed and YFP.

What is the difference between 470 nm and 505 nm LED transilluminators?

470 nm models are optimized for blue-light excitable stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green, while 505 nm models provide better excitation efficiency for longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.

Do I need an emission filter?

Yes. A suitable emission filter is recommended for clear fluorescence imaging. The best filter depends on the stain, camera and imaging workflow.

LED505-DF36W 505 nm Green LED Transilluminator for UV-Free Gel Imaging

Name: LED505-DF36W LED Transilluminator
Brand: Mecan USA
SKU: LED505-DF36W
Price: 950 USD
Availability: InStock

High-intensity dark-field LED transilluminator optimized for green-light excitation around 500 nm

The LED505-DF36W is a 505 nm green LED transilluminator designed for safe,
UV-free fluorescence gel imaging.

It provides efficient excitation for nucleic acid stains with absorption peaks around 490-510 nm, enabling high-sensitivity detection with dyes such as EtBr, GelRed and SYBR Gold.

This model is also compatible with selected protein stains and fluorescent proteins that respond to green-light excitation.

505 nm Green LED 36W Class Dark-Field Dichroic Filter UV-Free Gel Cutting

Features 470 vs 505 Specification Related FAQ

LED505-DF36W

505 nm visible green LED excitation for UV-free gel imaging.
Dark-field illumination helps reduce direct stray light into the camera or observer.
Dichroic filter system suppresses unwanted long-wavelength background light.
Suitable for DNA, RNA and electrophoresis gel observation, imaging and gel cutting.
Recommended for EtBr, GelRed, SYBR Gold and other longer-wavelength green-light excitable nucleic acid stains.

Set Composition & Price

LED Transilluminator with high-contrast sharp-cut filter cover, cuts off 560 nm or below.
AC-DC Adapter 100-240 V universal input, 24 V 2.7 A 65 W output.

Product	Item Code	Price
Dark-field LED Transilluminator, 505 nm green LED	LED505-DF36W	US$ 950

High-Intensity LED Arrangement

LED505-DF36W target illumination and light distribution

The LED505-DF36W uses 24 high-intensity chip-type LEDs arranged on both sides of the luminous surface.

This structure provides strong blue-light excitation while helping keep direct light away from the observer and camera.

Dark-Field Illumination System

The dark-field illumination system directs light from the lower left and right sides of the illuminator.

Because the excitation light does not directly enter the camera or observer, fluorescence can be observed with improved contrast.

Dark-field illumination system for LED transilluminator

Dichroic Filter Effect

The dichroic filter cuts unwanted long-wavelength components from the LED light.

Together with the light-absorbing mat, it helps suppress background glow and improve the visibility of weak fluorescence bands.

Removable Orange Observation Cover

The high-contrast orange sharp-cut cover can be attached and removed easily.

It allows observation and gel cutting while reducing excitation light below 560 nm.

LED transilluminator removable orange observation cover

Compatible with Pict LaBox and Bio-Pyramid Mini

LED505-DF36W with Pict LaBox gel documentation system

Pict LaBox Compatible

The LED505-DF36W can be combined with Pict LaBox for gel observation, cutting and image documentation using a digital camera or CCD camera system.

Bio-Pyramid Mini Compatible

The compact Bio-Pyramid Mini can be installed stably on the LED505-DF36W for mini-gel fluorescence imaging and documentation.

LED470 or LED505? Which Model Should You Choose?

Selection Guide Choose LED470-DF36W for blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. Choose LED505-DF36W for longer-wavelength green-light excitation dyes such as EtBr, GelRed and SYBR Gold. For protein stains or fluorescent proteins, please check the excitation and emission spectrum of each dye.

View LED470-DF36W View Filter Guide

Specification

Model	LED505-DF36W
Peak Wavelength	Green 505 nm
Luminous Area	140 x 130 mm effective area
LED	24 high-intensity chip-type LEDs arranged in parallel
Illumination Method	Dark-field illumination from diagonally below the left and right sides
Background Suppression	Multi-layer dichroic filters installed directly on the LED to suppress long-wavelength background light
Observation Cover Filter	Orange cast acrylic cover, cuts off 560 nm or below
Compatible Fluorescent Reagents	DNA / RNA stains optimized for ~500 nm excitation: EtBr, GelRed, SYBR Gold Protein stains compatible with green-light excitation: SYPRO Red, Flamingo Fluorescent proteins / markers: DsRed, YFP and similar fluorophores Recommended excitation range: Dyes with excitation or absorption peaks around 490-510 nm
Power Supply	Input: 100-240 V, 50/60 Hz universal / Output: 24 V, 2.7 A, 65 W / PSE, UL and CE certified
Power Consumption	32 W constant
LED Lifetime	Approx. 40,000 hours
Cooling	Forced-air cooling by fan
Accessories	AC-DC adapter, instruction manual
Body Size / Weight	W290 x D230 x H100 mm, H120 mm including cover Approx. 2.2 kg

What is LED505-DF36WLED505-DF36W is a 505 nm green LED transilluminator designed for safe, UV-free gel imaging. It uses dark-field illumination and a dichroic filter system to enhance fluorescence contrast for longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.

FAQ - LED505-DF36W

What is LED505-DF36W used for?	It is used for UV-free fluorescence excitation in DNA, RNA and electrophoresis gel imaging workflows using 505 nm green-light excitation, especially with longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.
Which stains are suitable for this model?	It is optimized for nucleic acid stains with excitation or absorption peaks around 490-510 nm, such as EtBr, GelRed and SYBR Gold. It is also compatible with selected protein stains such as SYPRO Red and Flamingo, and fluorescent proteins such as DsRed and YFP.
What is the difference between 470 nm and 505 nm models?	470 nm models are optimized for blue-light excitable stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green. 505 nm models provide better excitation efficiency for longer-wavelength dyes such as EtBr, GelRed and SYBR Gold.
Do I need an emission filter?	Yes. A suitable emission filter is recommended for clear fluorescence imaging. The best filter depends on the stain, camera and imaging workflow.

Need help selecting the right LED transilluminator? Please tell us your gel stain, gel size, camera system, emission filter and imaging workflow.
We will recommend a suitable wavelength and model configuration.