What is an LED illuminator for fluorescence excitation used for?

An LED fluorescence excitation illuminator provides selected blue or green excitation light for fluorescence observation with a stereo microscope, depending on the fluorescent reagent, sample and filter configuration.

What is the difference between transmitted and reflected fluorescence excitation?

Transmitted excitation illuminates the sample from below the stage, while reflected excitation illuminates the sample from above or from the side. The suitable method depends on sample transparency, fluorescence brightness, working distance and observation conditions.

Do fluorescence excitation systems require filters?

In many fluorescence observation setups, excitation and observation filters are used to improve contrast and reduce unwanted excitation light. Filter holder systems may be required depending on the microscope and application.

Microscope & Accessory

LED Illuminator for Fluorescence Excitation

LED transmitted and reflected-light excitation systems for stereo microscope fluorescence observation.

These LED excitation systems help enable fluorescence observation on a standard stereo microscope. Select transmitted excitation from below the stage, or reflected / twin-arm excitation from above or from the side, depending on the sample, reagent, working distance and filter configuration.

Typical applications include GFP observation, EtBr gel imaging, Cy3 fluorescence observation and general fluorescence screening workflows.

Blue / Green LED Excitation Transmitted Light Twin-Arm Epi Light Filter Holder Options

Transmitted Type Epi Type Filter Holders Selection Guide

LED Fluorescence Excitation Lineup

LED Transmitted Illuminator

TR8W Series

TR8W LED transmitted fluorescence excitation illuminator

Transmitted excitation

LED470-TR8W
LED505-TR8W

High-power LED transmitted illuminator for fluorescence excitation observation on a stereo microscope.

RT25 Series

RT25 LED transmitted fluorescence excitation illuminator

High-intensity transmitted type

RT25-B47-CR
RT25-G53-CR

High-intensity LED transmitted illuminator for fluorescence observation on a stereo microscope.

LED Epi-Illuminator

HDA Series

HDA LED twin-arm fluorescence excitation illuminator

Twin-arm epi excitation

HDA-TB3
HDA-TG3

LED twin-arm excitation light for reflected fluorescence observation with adjustable incident angle.

SDA-B4 / C4

SDA blue and green LED twin-arm fluorescence excitation illuminator

Compact twin-arm type

SDA-B4
SDA-C4

Blue or green LED twin-arm excitation type for compact fluorescence observation setups.

Filter Holder Systems

MFH-FL

Filter holder
Filter holder option for fluorescence and observation setups using compatible stereo microscopes.

LFH-FL

Large filter holder
Filter holder option for fluorescence observation setups where larger stage or base compatibility is required.

Filter Guide

Filter reference
Guide information for selecting filter combinations used in fluorescence observation workflows.

Selection Guide

Transmitted excitation

Use transmitted LED excitation when the sample or gel can be illuminated from below. This approach is useful for many transparent, translucent or gel-based fluorescence observation workflows.

Epi / reflected excitation

Use LED twin-arm excitation when lighting from above or from the side is preferred, especially when the incident angle needs to be adjusted or when the sample is not suitable for transmitted illumination.

Filter note: Fluorescence observation normally requires an appropriate excitation and observation filter combination. Actual visibility depends on the fluorescent reagent, excitation wavelength, sample brightness, microscope optics and ambient-light conditions.