Mecan LED Transilluminators are a UV-free alternative for gel imaging, using high-intensity monochromatic LEDs to visualize fluorescent bands with reduced UV exposure and sample damage.
Available in 470 nm (SYBR Green, GelGreen, Midori Green) and 505 nm (EtBr, GelRed, SYBR Gold) excitation models, in 36W and 60W configurations. Suitable for imaging, teaching labs and gel cutting with a compatible filter and documentation hood.
The 36W series is designed for routine gel imaging with high-intensity LED excitation and dark-field illumination. It is a practical choice for laboratories that need safe, clear and cost-effective fluorescence observation.
The 60W series uses direct illumination with chip-type LEDs positioned under the observation surface. This design provides strong excitation intensity for faint bands, high-sensitivity imaging and demanding fluorescence applications.
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| Model Type | Recommended Use | Typical Stains / Applications |
|---|---|---|
| 470 nm LED | Blue-light excitation for common safe DNA gel stains. | SYBR Green, SYBR Safe, GelGreen, Midori Green and similar blue-light excitable nucleic acid stains. |
| 505 nm LED | Green-light excitation for EtBr and red/orange fluorescence workflows. | EtBr, GelRed, SYBR Gold, SYPRO Red, Flamingo, DsRed, YFP and related longer-wavelength fluorescent dyes or markers. |
| 36W Series | Routine gel observation, imaging and gel cutting. | General laboratory use, teaching labs and standard documentation workflows. |
| 60W Series | High-sensitivity imaging when stronger excitation is required. | Faint bands, high-contrast imaging and demanding fluorescence observation. |
| Question | Answer |
|---|---|
| Can LED transilluminators replace UV transilluminators? |
For many fluorescent gel stains, yes. LED excitation is especially useful when UV-free observation, safer gel cutting and reduced sample damage are preferred. However, compatibility depends on the stain and filter combination. |
| Which model should I choose for SYBR Green or GelGreen? |
470 nm models are generally suitable for SYBR Green, SYBR Safe, GelGreen, Midori Green and similar blue-light excitable nucleic acid stains. |
| Which model should I choose for EtBr or GelRed? |
505 nm models are generally suitable for EtBr, GelRed, SYBR Gold and other longer-wavelength stains that respond well to green-light excitation around 490-510 nm. |
| Do I need an emission filter? | Yes, an appropriate emission filter is usually required for clear fluorescence imaging. The best filter depends on the stain, excitation wavelength and camera system. |