LED Transmitted Light for Fluorescence Excitation
Mecan LED transmitted light sources provide selected excitation wavelengths from below the specimen, gel or observation stage. The lineup includes compact transmitted LED light for stereo microscopes and 470 nm / 505 nm LED transilluminators for gel observation, fluorescence imaging and gel cutting workflows.
Choose the LED wavelength according to the fluorescent reagent, specimen, emission filter and camera system. For clear fluorescence observation, an appropriate emission filter or filter holder system is usually required.
LED Transmitted Light for Stereo Microscope
The TR8W Series is a compact transmitted LED light source for stereo microscope fluorescence excitation. It is suitable when a small, simple transmitted illumination unit is preferred for microscope observation or documentation.
High-contrast transmitted LED light for fluorescence excitation under stereo microscopes. Available wavelength variations include 470 nm blue LED and 505 nm cyan / green-region LED models.
LED Transilluminator - 36W High-Intensity Dark-Field Illumination
The DF36W Series provides 36W-class LED transmitted illumination for fluorescence gel observation and imaging. It is a practical choice for laboratories that need clear fluorescence excitation in a compact format.
Designed for blue-light excitation dyes such as SYBR Green, SYBR Safe, GelGreen, Midori Green and similar nucleic acid stains with excitation or absorption peaks around 450–490 nm.
Designed for longer-wavelength green-light excitation dyes such as EtBr, GelRed, SYBR Gold and related stains with excitation or absorption peaks around 490–510 nm.
LED Transilluminator - 60W High-Intensity Transmitted Illumination
The TR60W Series provides stronger LED excitation intensity for faint bands, high-sensitivity imaging and demanding fluorescence applications. Select 470 nm or 505 nm according to the stain, filter combination and imaging system.
High-output 470 nm excitation for weak fluorescence signals and high-sensitivity gel imaging with blue-light excitable nucleic acid stains such as SYBR Green, SYBR Safe, GelGreen and Midori Green.
High-output 505 nm excitation for EtBr, GelRed, SYBR Gold and other longer-wavelength fluorescence dyes where stronger excitation is preferred.
LED Transmitted Light for Microscopes and Fluorescence Workflows
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Selection Guide: 470 nm vs. 505 nm / 36W vs. 60W
| Model Type | Recommended Use | Typical Stains / Applications |
|---|---|---|
| 470 nm LED | Blue-light excitation for common safe DNA gel stains. | SYBR Green, SYBR Safe, GelGreen, Midori Green and similar blue-light excitable nucleic acid stains. |
| 505 nm LED | Green-light excitation for EtBr and red/orange fluorescence workflows. | EtBr, GelRed, SYBR Gold, SYPRO Red, Flamingo, DsRed, YFP and related longer-wavelength fluorescent dyes or markers. |
| 36W Series | Routine gel observation, imaging and gel cutting. | General laboratory use, teaching labs and standard documentation workflows. |
| 60W Series | High-sensitivity imaging when stronger excitation is required. | Faint bands, high-contrast imaging and demanding fluorescence observation. |
Please tell us your gel stain, gel size, camera system, emission filter and imaging workflow.
We can recommend a suitable wavelength and model configuration.