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How to Use a Stage Micrometer and an Ocular Micrometer
Together for Calibration

How to use Ocular Micrometer@How to use Stage Micrometer@How to install Ocular Micrometer
@@The Role of the Stage and Ocular Micrometers

I want to measure the size of the object,

@@@Should I use Ocular MicrometerH
@@@@Should I use Stage MicrometerH@

To put it simply,
An ocular micrometer is used to measure the size of objects
The stage micrometer is used to calibrate the ocular micrometer.
Making more accurate measurement possible with ocular micrometer.

The proper use of the stage micrometer and ocular micrometer is illustrated below
How to use Ocular Micrometer@How to use Stage Micrometer
1.@How to Use an Ocular Micrometer

Magnified object (by objective lens) is imaged on an ocular micrometer, enabling you to see the sample object and the scale at the same time. By knowing the actual length of one pitch of the ocular micrometer, you can measure the length of the sample.
The actual length of micrometer's each pitch changes according to the magnification of objective lens.
** Calculations are below.

Length of a pitch = mactual length of ocular micrometer's one pitchnmmagnification of objective lensn

Example of the Mesurement
E Below is an example of the measurement of a glomeruli of the kidney using an ocular micrometer (XY11).
The micrometer used in the picture has a pitch of 0.1mm (100m) and the objective lens used has a magnification ratio of 20.
E Using the calculation provided above one pitch corresponds to 5.0m (5m = 100m 20).
If the glomeruli spans 23 pitches on the horizontal axis and 22 pitches on the vertical axis,
it measures 115m (5m ~ 23) by 110m (5m ~ 22).

Magnification of
Objective Lens
Magnified Length of One Pitch
10mm/100 divisions, pitch 0.1mm(100m)
5~ @@@@@20m@@i=100m5j
10~ @@@@@10m@@i=100m10j
20~ @@@@@5m@@@i=100m20j
40~ @@@@@2.5m@@i=100m40j
50~ @@@@@2m@@@i=100m50j
100~ @@@@@1m@@@i=100m100j

How to use Ocular Micrometer@How to use Stage Micrometer
2.@How to use a Stage micrometer (calibrate the ocular micrometer for more accurate results)

The magnification of an objective lens isnft perfect. It is typically off by a small margin.
The purpose of the stage micrometer is to determine the error in magnification of the objective lens.
This allows the measurement of samples with greater precision.

E The first step is to correctly line up the stage and ocular micrometer (Make sure two are line up complete parallel)
Would calculate accurate magnification ration from error margin.
E The image below shows a stage micrometer NOB1 (10m pitch and 1mm/100 divisions) and
an ocular micrometer S11 (100m pitch and 10mm/100 divisions), with an objective lens magnification ratio at 20. B
E If magnification rate is correct, 10 pitchs of stage micrometer should be equal to20 pitchs (2000m) of
ocular micrometer (=[1 pitch of ocular micrometer is 10m] x 10 ptichs x [Objective lens x20] )
Instead, in below diagram, 10 pitchs of stage micrometer shows equals to 21 divisions of ocular micrometer,
therefore it can be calculated that magnification of objective lens is x21.
E To achieve the calibrated measurement of the sample, multiply the measured value of the sample by the labeled magnification of the objective lens divided by the actual magnification ratio.
(Ex. Calibrated measurement of sample = measurement of sample ~ labeled magnification ratio actual magnification ratio) (Calibration ratio = 20/21 = 0.95)

How to Install Ocular Micrometer
An ocular micrometer is a glass disk with ruled scale on the surface which is etched in chromium.
Please insert it into the eyepiece with written surface facing to objective lens.idownward on below imagej
About Size@@
Size of ocular micrometers depends on eyepiece lens. Please refer size list for Nikon and Olympus microscopes.@
Please contact the maker if your item is not listed, or if a microscope other than Nikon or Olympus is used..

scale | cross X |cross XY | section | all section/section XY | miller | concentric circle/cross line/grain scale | stage micrometer
eyepiece graticule for asbestos | ocular & stage micrometer pair set | micrometer for digital camera adapter


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