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Home >> Product >> Microscope >> Micrometer >> Calibration

How to Use Stage & Ocular Micrometer
Together for Calibration

How to use Ocular Micrometer@How to use Stage Micrometer@How to install Ocular Micrometer 
@@The Role of the Stage and Ocular Micrometers
I want to measure the size of the object,
@@@Should I use Ocular MicrometerH
@@@@Should I use Stage MicrometerH@
To put it simply
An ocular micrometer is used to measure the size of objects
The stage micrometer is used to calibrate the ocular micrometer.
Making more accurate measurement possible with ocular micrometer.


1.@How to Use an Ocular Micrometer
Magnified sample (by objective lens) is imaged on an ocular micrometer, enables us to see the sample and the micrometer scale at the same time when we see it through eyepiece. By determining ocular micrometer's pitch length under microscope, we can measure the sample size.
 The micrometer pitch length under microscope changes depending on objective magnification.
Micrometer Pitch Length (under microscope) =
= [Actual Micrometer Pitch LengthnmObjective Lens Magnificationn
* Example
E Below is an example of the measurement of a glomeruli of the kidney using an ocular micrometer XY11.
XY11 has a 0.1mm pitch (100m) and the objective lens has a 20x magnification.
E By using equation provided above, one pitch in this case corresponds to 5.0m (5m = 100m 20)
Therefore, If the Glomeruli spans 23 pitches on the micrometer's horizental scale and 22 pitches on the vertical scale,
it measures 115m (5m x 23) by 110m (5m x 22).
Objective Lens
Mag
Magnified Length of One Pitch
10mm/100 divisions, pitch 0.1mm(100m)
5 x 20m@( = 100m 5)
10 x 10m@( = 100m 10)
20 x 5m @@( = 100m 20)
40 x 2.5m@( = 100m 40j
50 x 2m@i= 100m 50j
100 x 1m@i= 100m 100j


2.@How to Use a Stage Micrometer (calibrate the ocular micrometer for more accurate results)
The magnification of an objective lens isn't perfect. It is typically off by a small margin.
The purpose of the stage micrometer is to determine the error in magnification of the objective lens.
This allows the measurement of samples with greater precision.

EXAMPLE OF THE DETERMINATION OF THE OBJECTIVE LENS MAGNIFICATION ERROR:@
E The first step is to correctly line up the stage and ocular micrometer (Make sure two are line up complete parallel)
Would calculate accurate magnification ration from error margin.
E The image below shows a stage micrometer NOB1 (10m pitch and 1mm/100 divisions) and
an ocular micrometer S11 (100m pitch and 10mm/100 divisions), with an objective lens magnification ratio at 20. B
E If magnification rate is correct, 10 pitchs of stage micrometer should be equal to20 pitchs (2000m) of
ocular micrometer (=[1 pitch of ocular micrometer is 10m] x 10 ptichs x [Objective lens x20] )
Instead, in below diagram, 10 pitchs of stage micrometer shows equals to 21 divisions of ocular micrometer,
therefore it can be calculated that magnification of objective lens is x21.
E To achieve the calibrated measurement of the sample, multiply the measured value of the sample by the labeled magnification of the objective lens divided by the actual magnification ratio.
(Ex. Calibrated measurement of sample = measurement of sample ~ labeled magnification ratio actual magnification ratio) (Calibration ratio = 20/21 = 0.95)



How to Install Ocular Micrometer
An ocular micrometer is a glass disk with ruled scale on the surface which is etched in chromium.
Please insert it into the eyepiece with written surface facing to objective lens (downward on below image)
@@@
By JIS standard, a small error margin is accepted for magnification of object lens. It is possible to determine the error margin and enables measurement with better accuracy by using Stage Micrometer, together
Image above is typical eyepiece lens. Ssize and shape may slightly different for each models.
@@** some of the unique designed eyepiece requires special treatment for insertion.








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