

How to use Stage & Ocular Micrometer
togother for Calibration

I want to measure the sample size under the microscope,
@@@Should I use Ocular MicrometerH
@@@@or Should I use Stage MicrometerH@ 


@To put it simply


An ocular micrometer is used for measuring the size of objects 

The stage micrometer is used for calibrating the ocular micrometer. 

(for more accurate measurement) 

@@How to Use an Ocular Micrometer 
When an ocular micrometer is set correctly inside the eyepiece, it should
enable to see a sample
and an micrometer scale simultaneously when you look through a microscope.
By determining
single pitch length of an micrometer scale, you will be able to measure the actual sample size. 
** Under the microscope, the single pitch length of an micrometer changes
as magnification of objective lens is changed.
Formula is :
Ocular Micrometer's Pitch Length (under the microscope)@=
[Actual Micrometer Pitch Lengthn@@mObjective Lens Magnificationn 

Below explains the measurement of a glomeruli (of the kidney) with following
two premises.
1. Ocular micrometer model XY11 is used. XY11 has 0.1mm pitch (100m)
2. Objective lens has a 20x magnification. 



Applying these numbers to above formula, one pitch of ocular micrometer
in this scenario
should be equal to 5.0m (= 100m 20). Since the glomeruli spans 23 pitches on the horizontal scale and 22 pitches on the vertical scale on micrometer,
it measures 115m (5m x 23) by 110m (5m x 22). 



Obj Lens
Mag 
Magnified Pitch Length
10mm /100 divisions / pitch 0.1mm is used 
5 x 
20m (= 100m 5) 
10 x 
10m (= 100m 10)

20 x 
5m (= 100m 20) 
40 x 
2.5m (= 100m 40j 
50 x 
2m (= 100m 50) 
100 x 
1m (= 100m 100) 

@@How to Use an Ocular & Stage Micrometer for Calibration 
Generally, the objective lens magnification is off by a small margin.
The purpose of the stage micrometer is to determine this subtle magnification
error in an objective lens.
The process below should enables measurement with greater precision. 

First, position stage and ocular micrometer in a way both scale are lined
up complete parallel
(to derive correct and accurate magnification from error margin) 



Below illustrate stage micrometer NOB1 (1mm/100 divisions = 10m pitch) and
an ocular micrometer S11 (10mm/100 divisions = 100m pitch).
objective lens magnification is at 20x. 



If magnification rate is correct, 10 pitches of stage micrometer should
be equal to 20 pitches (2000m) of an ocular micrometer (=[1 pitch of ocular
micrometer is 10m] x 10 pitches x [Objective lens x20] )Instead, in below
diagram, 10 pitches of stage micrometer shows equals to 21 divisions of
ocular micrometer,therefore it can be calculated that magnification of
objective lens is x21. 




To achieve the calibrated measurement of the sample, multiply the measured
value of the sample by the labeled magnification of the objective lens
divided by the actual magnification ratio.
(Ex. Calibrated measurement of sample = measurement of sample ~ labeled magnification ratio actual magnification ratio) (Calibration ratio = 20/21 = 0.95) 




@@@ 
Digital Camera Microscope Adapter & Special Micrometer S11CF 


NY1S
DSLR
Adapter 
Regular ocular micrometer has a scale and numbers etched at the center.
S11CF removed the numbers and shifted the scale from center for a clear
view !! 
