@@How to use Ocular & Stage Micrometer for Calibration

I want to measure the sample size under the microscope,
@@@Should I use Ocular MicrometerH
@@@@or Should I use Stage MicrometerH@
@To put it simply
An ocular micrometer is used for measuring the size of objects
The stage micrometer is used for calibrating the ocular micrometer.
(for more accurate measurement)

@@How to Use an Ocular Micrometer

When an ocular micrometer is set correctly inside the eyepiece, it should enable to see a sample
and an micrometer scale simultaneously when you look through a microscope. By determining
single pitch length of an micrometer scale, you will be able to measure the actual sample size.
** Under the microscope, the single pitch length of an micrometer changes
as magnification of objective lens is changed.
Formula is :
Pitch Length of Ocular Micrometer (under the microscope)@=
[Actual Micrometer Pitch Lengthn@@mObjective Lens Magnificationn

Example :
Below explains the measurement of a glomeruli (of the kidney) with following two premises.
1. Ocular micrometer model XY11 is used. XY11 has 0.1mm pitch (100m)
2. Objective lens has a 20x magnification.
Applying these numbers to above formula, one pitch of ocular micrometer in this scenario
should be equal to 5.0m (= 100m 20). Since the glomeruli spans 23 pitches on the horizontal scale and 22 pitches on the vertical scale on micrometer,
it measures
115m (5m x 23) by 110m (5m x 22).

Obj Lens
Magnified Pitch Length
10mm /100 divisions / pitch 0.1mm is used
5 x 20m (= 100m 5)
10 x 10m (= 100m 10)
20 x 5m (= 100m 20)
40 x 2.5m (= 100m 40j
50 x 2m (= 100m 50)
100 x 1m (= 100m 100)

@@How to Use an Ocular & Stage Micrometer for Calibration

Generally, the objective lens magnification is off by a small margin.
The purpose of the stage micrometer is to determine this subtle magnification error in an objective lens.
The process below should enables measurement with greater precision.
Example :
First, position stage and ocular micrometer in a way both scale are lined up complete parallel
(to derive correct and accurate magnification from error margin)
Below illustrate stage micrometer NOB1 (1mm/100 divisions = 10m pitch) and
an ocular micrometer
S11 (10mm/100 divisions = 100m pitch).
objective lens magnification is at 20x.
If magnification rate is correct, 10 pitches of stage micrometer should be equal to 20 pitches (2000m) of an ocular micrometer (=[1 pitch of ocular micrometer is 10m] x 10 pitches x [Objective lens x20] )Instead, in below diagram, 10 pitches of stage micrometer shows equals to 21 divisions of ocular micrometer,therefore it can be calculated that magnification of objective lens is x21.
To achieve the calibrated measurement of the sample, multiply the measured value of the sample by the labeled magnification of the objective lens divided by the actual magnification ratio.
(Ex. Calibrated measurement of sample = measurement of sample ~ labeled magnification ratio actual magnification ratio) (Calibration ratio = 20/21 = 0.95)

How to Install Ocular Micrometer
An ocular micrometer is a glass disk with ruled scale on the surface which is etched in chromium.
Please insert it into the eyepiece with written surface facing to objective lens (downward on below image)
By JIS standard, a small error margin is accepted for magnification of object lens. It is possible to determine the error margin and enables measurement with better accuracy by using Stage Micrometer, together
Image above is typical eyepiece lens. Ssize and shape may slightly different for each models.
@@** some of the unique designed eyepiece requires special treatment for insertion.

@ How to identify front and reverse side of ocular micrometer.
@Micrometer (scale and numbers) is printed on the glass with chrome.
@When printed side is identified, insert ocular micrometer to eyepiece fixing bracket
@with printed side facing downward (toward objective lens - refer also to above installing instruction)
@Here explains how to identify front (printed side) and reverse side.

 1. Shed light on the micrometer and
  let it reflects in a way that printed
  numbers and scale will be
  turned silver.

Unable to identify sides without
the reflection of light.
** To generate enough reflection, large area lighting is recommended
** Eyepieces lens can be used as loupe and it helps you for installation

 2. When there is no shade,
  it is reverse side

 2. When there is shade,
  it is fron side (printed side)